绿帽换妻

Specific research questions we are currently pursuing are:

• How can some plants grow where other plants can not?

• How do plant roots and shoots communicate with each other?

• Do plants have a memory, and can they learn?

• How can some plants live forever?

To explore these research questions, we are using a wide variety of experimental approaches that include next-generation sequencing, genome editing with CRISPR-Cas, qRT-PCR, proteomics, bioinformatics, and plant learning set-ups. If you are interested in our research, feel free to e-mail me!

• PhD, University of Bielefeld, Germany

Uhde-Stone, C. (2017). White lupin: a model system for understanding plant adaptation to low phosphorus availability Legume Nitrogen Fixation in Soils with Low Phosphorus Availability (pp. 243-280): Springer.

Amadi, M., Cole, J., Li, K., Chalkley, R. J., Burlingame, A., Uhde-Stone, C. (2017). Unraveling White Lupin’s Signal Transduction in Response to Phosphorus Deficiency Using iTRAQ Labeling, Phosphopeptide Enrichment, and Tandem Mass Spectrometry. The FASEB Journal, 31(1_supplement), 617.611-617.611.

Afshari A, Uhde-Stone C, Lu B. (2015). A cooled CCD camera-based protocol provides an effective solution for in vitromonitoring of luciferase. Biochem Biophys Res Commun. 458(3):543-8.

Afshari A, Uhde-Stone C, Lu B. (2014) Live visualization and quantification of pathway signaling with dual fluorescent and bioluminescent reporters. Biochem Biophys Res Commun. 448(3):281-6.

Uhde-Stone C, Sarkar N, Antes T, Otoc N, Kim Y, Jiang YJ, Lu B. (2014). A TALEN-based strategy for efficient bi-allelic miRNA ablation in human cells. RNA. 2014 Jun; 20(6):948-55.

Uhde-Stone C, Cheung E, Lu B.(2014) TALE activators regulate gene expression in a position- and strand-dependent manner in mammalian cells. Biochem Biophys Res Commun. 443(4):1189-94.

Uhde-Stone C, Gor N, Chin T, Huang J, Lu B.(2013). A do-it-yourself protocol for simple transcription activator-like effector assembly. Biol Proced Online. 15(1):3.

O'Rourke JA, Yang SS, Miller SS, Bucciarelli B, Liu J, Rydeen A, Bozsoki Z, Uhde-Stone C, Tu ZJ, Allan D, Gronwald JW, Vance CP. (2013). An RNA-Seq transcriptome analysis of orthophosphate-deficient white lupin reveals novel insights into phosphorus acclimation in plants. Plant Physiol. 161(2):705-24.

Uhde-Stone C, Huang J, Lu B. (2012) A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors. Biol Proced Online. 8;14(1):8.

Liu, M., Uhde-Stone, C., and C. Goudar. 2011. Progress curve analysis of qRT-PCR reactions using the logistic growth equation. Biotechnol. Prog.27: 1407-1414.

Rath, M., Salas, J., Parhy, B., Norton, R., Hatlstad, G., Kwon, J., Allan, D.L., C.P. Vance, and C. Uhde-Stone. 2010. Identification of genes induced in proteoid roots of white lupin under nitrogen and phosphorus deficiency, with functional characterization of a formamidase. Plant and Soil334:137-150

Norton, R., Uhde-Stone, C., and M. Rath. 2007. Array analysis to assess effects of RNA_i-based silencing. JSM Proceedings, Biometrics Section [CD-ROM], Alexandria, VA: American

Statistical Association: 263-268

Uhde-Stone, C., Liu J., Zinn, K. E., Vance C.P., and D. L. Allan. 2005.Transgenic proteoid roots of white lupin: a vehicle for characterizing and silencing root genes involved in

adaptation to P stress.Plant Journal 44:840-853

Liu, J., Yamagishi, M., Uhde-Stone, C., Bucciarelli, B., Samac, D.A., Allan, D., and C.P.

Vance. 2005. Shoot signals and molecular regulation of P-deficiency induced genes in cluster

roots of white lupin. In: Plant Nutrition for Food Security, Human Health and Environmental Protection. Tsinghua University Press. p 94-96

Uhde-Stone, C., Zinn, K.E., Ramirez-Yánez, M., Li, A., Vance C.P. and D.L. Allan. 2003.

Nylon filter arrays reveal differential gene expression in proteoid roots of white lupin in

response to P deficiency. Plant Physiology 131:1064-1079.

Vance, C.P., Uhde-Stone, C., and D.L. Allan. 2003. Phosphorus acquisition and use: critical

adaptations by plants for securing a nonrenewable resource. Tansley Review. New

Phytologist 157:423-447.

Uhde-Stone, C., Gilbert, G., Johnson, J. M.-F, Litjens, R., Zinn, K.E., Temple, S.J., Vance,

C.P. and D.L. Allan. 2003. Acclimation of white lupin to phosphorus deficiency involves

enhanced expression of genes related to organic acid metabolism. Plant and Soil 248:99-116.

Uhde-Stone, C., Li, A., Daemen, M., Allan, D.L., and C.P. Vance. 2001. Isolation and

characterization of white lupin proteoid root expressed sequence tags (ESTs) associated with

plant hormones. p.32-33 InDevelopment in Plant and Soil Sciences: Plant Nutrition. Food

security and sustainability of agro-ecosystems through basic and applied research, Kluwer

Academic Publishers.

Liu, J., Uhde-Stone, C., Li, A., Vance, C.P., and D.L. Allan. 2001. A phosphate transporter

with enhanced expression in proteoid roots of white lupin (Lupinus albusL.). Plant and Soil

237:257-266.

Uhde, C.,Schmidt, R., Jording, D., Selbitschka, W., and A. Pühler. 1997. Stationary phase

mutants of Sinorhizobium melilotiare impaired in stationary phase survival

or in recovery to logarithmic growth. Journal of Bacteriology 179:6432-6440.

Jording, D.,Uhde, C., Schmidt, R., and A. Pühler. 1994. The C₄-dicarboxylate transport system

ofRhizobium melilotiand its role in nitrogen fixation during symbiosis with alfalfa (Medicago

sativa). InExperientia 50. Birkhäuser Verlag, CH-4010 Basel, Switzerland. 874-883.

Jording, D., Sharma, P.K., Schmidt, R., Engelke, T., Uhde, C.,and A. Pühler.1993. Regulatory

aspects of the C₄-dicarboxylate transport in Rhizobium meliloti: transcriptional activation

and dependence on effective symbiosis. Journal of Plant Physiology 141:18-27.